Glossary

Key Points

Introduction to the bioinformatic analysis of eDNA metabarcoding data	Most metabarcoding pipelines include the same basic components Frequency tables are key to metabarcoding analyses
Demultiplexing and Trimming	First key point. Brief Answer to questions. (FIXME)
Quality filtering	Quality filtering on sequence length, ambiguous basecalls, and maximum error rate
OTU clustering	Clustering sequences into OTUs can miss closely related species Denoising sequences can create overestimate diversity when reference databases are incomplete
Creating A Reference Database	First key point. Brief Answer to questions. (FIXME)
Taxonomy Assignment	The main taxonomy assignment strategies are global alignments, local alignments, and machine learning approaches The main difference is that Naive Bayes requires the reference data to be trained, whereas Sintax looks for only kmer-based matches
Statistics and Graphing with R	A basic tutorial to conduct statistical analyses of your data

FIXME