Glossary

Key Points

Introduction to the bioinformatic analysis of eDNA metabarcoding data	Most metabarcoding pipelines include the same basic components Frequency tables are key to metabarcoding analyses
Sequencing data preparation	First key point. Brief Answer to questions. (FIXME)
Bioinformatic analysis	Quality filtering on sequence length, ambiguous basecalls, and maximum error rate Clustering sequences into OTUs can miss closely related species Denoising sequences can create overestimate diversity when reference databases are incomplete
Taxonomy assignment	The main taxonomy assignment strategies are global alignments, local alignments, and machine learning approaches Local curated reference databases increase taxonomic assignment accuracy
Data curation	The presence of artefacts inflate diversity metrics, but can be removed using novel algorithms The need for rarefaction can be tested using several methods
Statistics and Graphing with R	A basic tutorial to conduct statistical analyses of your data

FIXME